Multi-Mode Microplate Reader LMMR-A10

The reader uses a UV Xenon flashlamp as its light source. This type of lamp ensures a stable, reliable, and consistent energy output across the full wavelength range of 200–1000 nm, supporting accurate and repeatable measurements in absorbance, fluorescence, and luminescence assays.

Yes, the instrument offers advanced temperature control, ranging from room temperature +4 °C up to 45 °C. It maintains a very stable environment with an accuracy of ±0.5 °C at 37 °C. This precise temperature regulation is critical for assays that require consistent incubation conditions.

For absorbance measurements, the system uses a UV-silicon photodiode detector and covers a wavelength range of 200–1000 nm. The wavelength accuracy is ±0.5 nm for wavelengths greater than 315 nm and ±0.3 nm for 315 nm or below. Similarly, wavelength reproducibility is ±0.5 nm above 315 nm and ±0.3 nm at or below 315 nm. The reader provides a dynamic range of 0–4 OD, with high precision at 260 nm (CV <1.0% or SD <0.003) and accuracy of ±(1.0% + 0.003 Abs) at 0–2.0 Abs. It also offers ratio accuracy of ±0.07 at 260/280 nm.

For fluorescence measurements, the reader uses a photomultiplier tube (PMT) detector that is UV- and red-sensitive, ensuring detection across a wide spectrum. The excitation wavelength range is 200–1000 nm, and the emission range is 270–850 nm. It achieves high sensitivity with bottom detection as low as 5 amol per well and an intensity detection limit of 45 PM. Fluorescence polarization is supported with sensitivity at 1 nM (SD <4 m), and FP detection is accurate up to 5 mP. Bandwidth control is also provided: excitation bandwidth is <5 nm at ?315 nm and <9 nm above 315 nm, while emission bandwidth is <20 nm. Wavelength accuracy is ±2.0 nm for >315 nm and ±1.0 nm for ?315 nm, with reproducibility of ±1.0 nm and ±0.5 nm respectively.

The LMMR-A10 provides multiple shaking modes to suit different assay requirements. It supports linear shaking, annular shaking, and double annular shaking modes. These features ensure proper sample mixing, uniform reaction conditions, and reliable assay outcomes.

Yes, the system is optimized for sensitive detection in low-volume samples. It supports DNA detection down to 1 ng/µl in low-volume conditions, which makes it suitable for applications in molecular biology and genomics where sample amounts are often limited.

The instrument is fully controlled by software, allowing automated operation, including opening and closing of the system. It comes with ReaderIt-II software, which is 21 CFR Part 11 compliant. This ensures secure data handling, audit trails, and compliance with regulatory requirements for laboratories working under GLP/GMP standards. Additionally, it is compatible with external PCs (Win7/Win10 with Core i5 or higher) and printers for flexible data management.

The LMMR-A10 includes optional compatibility with automated liquid handling through injector blocks, allowing future scalability. Installation, qualification, and onsite training are typically provided, even though not explicitly listed in the catalog, ensuring smooth adoption in the lab. The system also provides broad connectivity and expandability options, making it a future-ready solution for modern laboratory workflows.

Yes, the LMMR-A10 supports absorbance, fluorescence, and luminescence assays, making it highly versatile for different experimental needs. It uses an advanced monochromator system for wavelength selection, which allows coverage from 200 to 1000 nm. This system provides two excitation and two emission options, ensuring precise wavelength selection for different assays.

The LMMR-A10 is designed to support a wide variety of microplates ranging from 6 wells up to 384 wells, including standard 96-well plates. It is also capable of reading a full 96-well plate in less than 15 seconds, which makes it efficient for high-throughput applications.

A Microplate Reader, commonly called as plate reader or plate spectrophotometer, is an analytical instrument that reads or detects the physical, chemical, and biological events of the analyte, within the wells of the plate. It has numerous tiny wells where the sample analyte is introduced for analysis.

A standard spectrophotometer measures the absorbance of one sample at a time. The sample is typically placed in a cuvette through which light is sent horizontally. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well vertically.

Microplate-based measurements detect light signals produced by a sample, converted by a sample, or transmitted through a sample. In the plate reader, the signal is measured by a detector, usually a photomultiplier tube (PMT). PMTs convert photons into electricity that is then quantified by the microplate reader. The output of this process is numbers by which a sample is quantified.

Tungsten-halogen or xenon flash lamps are generally used. LEDs have also come into the picture in some modern readers.

The detection technology can be of 3 types- Monochromator based detection which provides flexibility, convenience, and spectral scanning; Filter based detection which enables rapid switch between different wavelengths; Hybrid detection which has dual technological advantage of both flexibility and sensitivity.